Live-cell imaging
We observe cellular dynamics in intact, living islets.
Our imaging toolbox
Calcium sensors and dyes
Cilia-targeted reporters
Glucose and metabolic sensors
Genetic reporter mice
Adenoviral transductions
Microscopes
Nikon AXR NSPARC (WashU WUCCI)
Zeiss LSM880 (WashU WUCCI)
Live-cell applications
We use biosensors and dyes delivered to islet cells to study their real-time responses to glucose, hormones, and drugs.
Calcium signaling in islets
cilia (green), calbryte (red)
PKA signaling in beta cells
eXrai-akar2
Cilia waveform recordings
sstr3-gfp
SSTR3 in beta-delta cell crosstalk
gpcr signaling